Interview with Prof Alessandro Porchetta

New interview with Prof Alessandro Porchetta relating to the recent publication on Angew Chem

#Cas12,  #CRISPR  biosensors, #strand displacement reaction


New Paper in Angewandte Chemie International Edition

New paper in Angewandte Chemie International Edition

PAM-Engineered Toehold Switches as Input-Responsive Activators of CRISPR-Cas12a for Sensing Applications

Toehold Switch DNA presenting a locked anti-PAM in the loop are designed to control Cas12a activity in response to different molecular  inputs.  The hairpin  to  duplex  reconfiguration  triggered  by  co-localization-based  displacement  reactions  provides  a means to control the accessibility of the PAM and as a consequence Cas12a activity. We demonstrate the rapid, one-pot detection of antibodies and small molecules with high sensitivity and specificity.

#Cas12,  #CRISPR  biosensors, #strand displacement reaction

Paper: on this publication are available on AMS Acta repository

New Paper in Chemical Communication

New paper in Chemical Communication

Electrogenerated chemiluminescence at boron-doped diamond electrodes

The paper presents Boron Doped Diamond as new electrode for Electrochemiluminescence:

Boron-Doped Diamond (BDD) as an electrode material for ECL which has complementary properties compared to the most common metals (e.g., Au or Pt) and carbon materials (e.g., glassy carbon, carbon nanotubes and graphene). Boron-doped diamond electrodes emerged as novel electrodes, gaining more and more interest from the electrochemical community for their peculiar characteristics such as a wide solvent window, low capacitance, resistance to fouling and mechanical robustness.

Paper: Chemical Communication BDD ECL


Publication by Davide Migliorelli on Analytical and Bioanalytical Chemistry

“Design of a printed electrochemical strip towards miRNA‑21 detection in urine samples: optimization of the experimental procedures for real sample application” Wanda Cimmino, Davide Migliorelli, Sima Singh, Antonella Miglione, Silvia Generelli, Stefano Cinti
Analytical and Bioanalytical Chemistry.

Read the articles