New Paper in Analytical and Bioanalytical Chemistry

New Paper in Analytical and Bioanalytical Chemistry

Electrogenerated chemiluminescence from luminol-labelled microbeads triggered by in situ generation of hydrogen peroxide

A. Fiorani, C. Ignazio Santo, K. Sakanoue, D. Calabria, M. Mirasoli, F. Paolucci, G. Valenti, Y. Einaga

We developed a sensing strategy that mimics the bead-based electrogenerated chemiluminescence immunoassay. However, instead of the most common metal complexes, such as Ru or Ir, the luminophore is luminol. The electrogenerated chemiluminescence of luminol was promoted by in situ electrochemical generation of hydrogen peroxide at a boron-doped diamond electrode. The electrochemical production of hydrogen peroxide was achieved in a carbonate solution by an oxidation reaction, while at the same time, microbeads labelled with luminol were deposited on the electrode surface. For the first time, we proved that was possible to obtain light emission from luminol without its direct oxidation at the electrode. This new emission mechanism is obtained at higher potentials than the usual luminol electrogenerated chemiluminescence at 0.3–0.5 V, in conjunction with hydrogen peroxide production on boron-doped diamond at around 2–2.5 V (vs Ag/AgCl).

#electrochemiluminescence,  #Microscopy, Electrode,  biosensors

Paper: https://link.springer.com/article/10.1007/s00216-024-05356-zData on this publication are available on AMS Acta repository

Nano-immunoEra mid-term meeting in Rome

🌎Nano-immunoEra mid-term meeting in Rome: Happy to share some moments of the workshop organized by the UNITOV analytical group.

Some pictures

Nano-immunoEra workshop (14-17 April 2024 ) in Bologna

🌎Nano-immunoEra workshop (14-17 April 2024 ) at UNIBO: Happy to share some moments of the workshop organized by the UNIBO analytical group. The workshop “One health: new frontiers for bioanalytical chemistry” lasted two and a half days accompanied by a second event focused on “quality control workshop for the one health challenge: the quality of the data”. Particular attention was on the challenges of Data Management in One Health Model and the concept of FAIR data

Some pictures

Interview with Prof Alessandro Porchetta

New interview with Prof Alessandro Porchetta relating to the recent publication on Angew Chem

https://onlinelibrary.wiley.com/doi/10.1002/anie.202319677

#Cas12,  #CRISPR  biosensors, #strand displacement reaction

Interview

New Paper in Angewandte Chemie International Edition

New paper in Angewandte Chemie International Edition

PAM-Engineered Toehold Switches as Input-Responsive Activators of CRISPR-Cas12a for Sensing Applications

Toehold Switch DNA presenting a locked anti-PAM in the loop are designed to control Cas12a activity in response to different molecular  inputs.  The hairpin  to  duplex  reconfiguration  triggered  by  co-localization-based  displacement  reactions  provides  a means to control the accessibility of the PAM and as a consequence Cas12a activity. We demonstrate the rapid, one-pot detection of antibodies and small molecules with high sensitivity and specificity.

#Cas12,  #CRISPR  biosensors, #strand displacement reaction

Paper: https://doi.org/10.1002/anie.202319677Data on this publication are available on AMS Acta repository

Meet our secondees Elisa Paialunga

🌎Meet our secondees: Elisa Paialunga from 🇮🇹 (UNITOV) to 🇨🇭(CSEM). Elisa Paialunga will work on the design and fabrication of microfluidic systems through 3D printing.

🇮🇹 => 🇨🇭

Elisa Paialunga obtained a master’s degree in Chemistry from the University of Rome, Tor Vergata, under the supervision of Prof. Alessandro Porchetta. Currently, she is in the final year of her Ph.D. in Chemical Sciences & Technologies, under the supervision of Prof. Laura Micheli.

 

Her research focuses on the development of biosensors based on CRISPR-Cas for the detection of antibodies.

 

In Nano-ImmunoEra, she is actively involved in the design and fabrication of microfluidic systems through 3D printing, enabling the transposition of CRISPR-Cas assays into diagnostics in integrated devices.


Nano-ImmunoEra online Meeting 11/01/2024

Jan 11st, 2024. NanoImmunoERA online meeting for the MSCA Staff Exchanges: sharing the results of the first year of the project. 🇮🇹🇪🇸🇳🇱🇩🇪🇺🇸🇿🇦🇨🇭

Agenda

Meet our secondees Andrea Celeste Di Pede

🌎Meet our secondees: Andrea Celeste Di Pede from 🇮🇹 (UNITOV) to  🇳🇱 (WUR). Andrea will work on the development of CRISPR-Cas based biosensors for antibody detection.

🇮🇹 => 🇳🇱

Andrea Celeste obtained a Master’s degree in Chemistry at the University of Tor Vergata. She is currently doing a PhD in Chemical Science under the supervision of Prof. Alessandro Porchetta.

The research focuses on the development of CRISPR-Cas systems for diagnostics of nucleic acids.

In Nano-ImmunoEra she is involved in the development of CRISPR-Cas based biosensors for antibody detection.


Meet our secondees Neda Bagheri

🌎Meet our secondees: Neda Bagheri from 🇮🇹 (UNITOV) to 🇩🇪 (INNE). Neda Bagheri will work on the development of CRISPR-based POC platforms for the monitoring of antibodies

🇮🇹 => 🇩🇪

Neda is currently pursuing a PhD at the University of Rome Tor Vergata in the field of chemical science under the supervision of Prof. Alessandro Porchetta.

 

Neda’s research focuses on recreating nature-inspired strategies to program CRISPR-Cas systems using innovative DNA nanodevices.

In Nano-ImmunoEra she contributes to the development of CRISPR-based POC platforms for the monitoring of antibodies.


Nano-immunoEra Video is now online

🌎Nano-immunoEra video: Nano-ImmunoEra is a research and innovation staff exchange network aiming at the development of innovative biosensors and diagnostic tools for the detection of clinically relevant antibodies. Nano-ImmunoEra will form the next generation of clinically-oriented analytical scientists capable of delivering innovative solutions that will improve the lives of patients, reduce the cost of healthcare and position Europe as a leader in biomedical devices.

See the video in our youtube page