New Paper in Angewandte Chemie International Edition

New paper in Angewandte Chemie International Edition

PAM-Engineered Toehold Switches as Input-Responsive Activators of CRISPR-Cas12a for Sensing Applications

Toehold Switch DNA presenting a locked anti-PAM in the loop are designed to control Cas12a activity in response to different molecular  inputs.  The hairpin  to  duplex  reconfiguration  triggered  by  co-localization-based  displacement  reactions  provides  a means to control the accessibility of the PAM and as a consequence Cas12a activity. We demonstrate the rapid, one-pot detection of antibodies and small molecules with high sensitivity and specificity.

#Cas12,  #CRISPR  biosensors, #strand displacement reaction

Paper: https://doi.org/10.1002/anie.202319677

Meet our secondees Elisa Paialunga

🌎Meet our secondees: Elisa Paialunga from 🇮🇹 (UNITOV) to 🇨🇭(CSEM). Elisa Paialunga will work on the design and fabrication of microfluidic systems through 3D printing.

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Elisa Paialunga obtained a master’s degree in Chemistry from the University of Rome, Tor Vergata, under the supervision of Prof. Alessandro Porchetta. Currently, she is in the final year of her Ph.D. in Chemical Sciences & Technologies, under the supervision of Prof. Laura Micheli.

 

Her research focuses on the development of biosensors based on CRISPR-Cas for the detection of antibodies.

 

In Nano-ImmunoEra, she is actively involved in the design and fabrication of microfluidic systems through 3D printing, enabling the transposition of CRISPR-Cas assays into diagnostics in integrated devices.


Nano-ImmunoEra online Meeting 11/01/2024

Jan 11st, 2024. NanoImmunoERA online meeting for the MSCA Staff Exchanges: sharing the results of the first year of the project. 🇮🇹🇪🇸🇳🇱🇩🇪🇺🇸🇿🇦🇨🇭

Agenda

Meet our secondees Andrea Celeste Di Pede

🌎Meet our secondees: Andrea Celeste Di Pede from 🇮🇹 (UNITOV) to  🇳🇱 (WUR). Andrea will work on the development of CRISPR-Cas based biosensors for antibody detection.

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Andrea Celeste obtained a Master’s degree in Chemistry at the University of Tor Vergata. She is currently doing a PhD in Chemical Science under the supervision of Prof. Alessandro Porchetta.

The research focuses on the development of CRISPR-Cas systems for diagnostics of nucleic acids.

In Nano-ImmunoEra she is involved in the development of CRISPR-Cas based biosensors for antibody detection.


Meet our secondees Neda Bagheri

🌎Meet our secondees: Neda Bagheri from 🇮🇹 (UNITOV) to 🇩🇪 (INNE). Neda Bagheri will work on the development of CRISPR-based POC platforms for the monitoring of antibodies

🇮🇹 => 🇩🇪

Neda is currently pursuing a PhD at the University of Rome Tor Vergata in the field of chemical science under the supervision of Prof. Alessandro Porchetta.

 

Neda’s research focuses on recreating nature-inspired strategies to program CRISPR-Cas systems using innovative DNA nanodevices.

In Nano-ImmunoEra she contributes to the development of CRISPR-based POC platforms for the monitoring of antibodies.


Nano-immunoEra Video is now online

🌎Nano-immunoEra video: Nano-ImmunoEra is a research and innovation staff exchange network aiming at the development of innovative biosensors and diagnostic tools for the detection of clinically relevant antibodies. Nano-ImmunoEra will form the next generation of clinically-oriented analytical scientists capable of delivering innovative solutions that will improve the lives of patients, reduce the cost of healthcare and position Europe as a leader in biomedical devices.

See the video in our youtube page

Nano-immunoEra workshop (13-15 December 2023 ) in Rome

🌎Nano-immunoEra workshop (13-15 December 2023 ) at UNITOV: Happy to share some moments of the workshop organized by the UNITOV analytical group. The workshop was attended by 10 researchers and secondees of Nano-immunoEra showing the results of the project to the sensor community.

Some pictures

2023 European Researchers' Night: Madrid

Last week in Madrid we participated at the European Researchers Night 2023 showing the results of the NanoImmunoERA project

Some pictures

2023 European Researchers' Night: Bologna

Yesterday our group of researchers from Bologna participated in the researchers’ night 2023 showing the results of the NanoImmunoERA project

Some pictures

Meet our secondees Elisa Lazzarini

🌎Meet our secondees: Elisa Lazzarini (UNIBO) from 🇮🇹  to  🇳🇱 (WUR). Elisa will work on chemiluminescence and electrochemiluminescence investigation of CRISPR-Cas based biosensors

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Elisa obtained a Master’s degree in Molecular and Industrial Biotechnology at the University of Bologna, she is currently doing a PhD in Nanoscience for Medicine and the Environment under the guidance of Prof. Mara Mirasoli.

 

The research focuses on the development of new portable devices for multiplexed detection based on chemiluminescent enzyme assays, immunoassays or gene probe assays, using microfabrication technology and microfluidics for the quantitative analysis of molecules of interest in the medical, alimentary and environmental fields.

In Nano-ImmunoEra she is involved in the electrochemiluminescence investigation of CRISPR-Cas based biosensors