Meet our secondees Alenzo Murray
🌎Meet our secondees: Alenzo Murray from the University of Western Cape, South Africa 🇿🇦 to the University of Bologna, Italy 🇮🇹.
🇿🇦 => 🇮🇹
Alenzo is currently a PhD student at the University of Western Cape, South Africa in the field of electrochemistry and developing chemical- and biosensors systems for biomedical applications under the supervision of Prof. Priscilla Baker
His research focuses on developing biosensors for the detection of prostate cancer biomarkers such as spermine and spermidine in urine.
His research aims to improve the sensor’s performance for early diagnosis and screening of prostate cancer by modifying the electrode surfaces with two-dimensional conductive metal-organic framework.]
Meet our secondees Alejandro Chamorro
🌎Meet our secondees: Alejandro Chamorro from 🇮🇹 UNITOV to 🇺🇸 UCSB. Alejandro will work on aptamer based biosensors for the monitoring of monoclonal therapeutic antibodies for cancer treatments.
🇮🇹 => 🇺🇸
Alejandro is currently a postdoctoral researcher at the University of Rome Tor Vergata in the field of analytical science collaborating with Prof. Alessandro Porchetta.
Alejandro’s research focuses on developing aptamer based biosensors (EAB sensors) for the detection of therapeutical drugs.
In Nano-ImmunoEra he contributes to the development of EAB sensors platforms for the monitoring of monoclonal therapeutic antibodies for cancer treatments.
Interview with Melisa del Barrio (UCM)
New interview with Melisa del Barrio (Secondee UCM)
#analytical chemistry, #Cas12, #CRISPR biosensors, #strand displacement reaction
Interview with Pablo Purohit (UCM)
New interview with Pablo Purohit (Secondee UCM)
#analytical chemistry, #Cas12, #CRISPR biosensors, #strand displacement reaction
Meet our secondees Claudio Ignazio Santo
🌎Meet our secondees: Claudio Ignazio Santo from 🇮🇹 (UNIBO) to🇩🇪(INNE). Claudio will work on innovative methods for antibody detection based on the combination of CRISP and lateral flow.
🇮🇹 => 🇩🇪
Claudio is currently pursuing a PhD in chemistry at the University of Bologna in the field of electrochemistry under the supervision of Prof. Francesco Paolucci and Prof. Giovanni Valenti.
Claudio’s research focuses on Electrochemiluminescence and its optimization for biosensors’ application.
In the Nano-ImmunoEra project, he contributes by analyzing and developing innovative methods for antibody detection. Simultaneously, he focuses on the characterization and optimization of novel materials for enhanced electrochemiluminescence (ECL) applications.
New Paper in Analytical and Bioanalytical Chemistry
New Paper in Analytical and Bioanalytical Chemistry
Electrogenerated chemiluminescence from luminol-labelled microbeads triggered by in situ generation of hydrogen peroxide
A. Fiorani, C. Ignazio Santo, K. Sakanoue, D. Calabria, M. Mirasoli, F. Paolucci, G. Valenti, Y. Einaga
We developed a sensing strategy that mimics the bead-based electrogenerated chemiluminescence immunoassay. However, instead of the most common metal complexes, such as Ru or Ir, the luminophore is luminol. The electrogenerated chemiluminescence of luminol was promoted by in situ electrochemical generation of hydrogen peroxide at a boron-doped diamond electrode. The electrochemical production of hydrogen peroxide was achieved in a carbonate solution by an oxidation reaction, while at the same time, microbeads labelled with luminol were deposited on the electrode surface. For the first time, we proved that was possible to obtain light emission from luminol without its direct oxidation at the electrode. This new emission mechanism is obtained at higher potentials than the usual luminol electrogenerated chemiluminescence at 0.3–0.5 V, in conjunction with hydrogen peroxide production on boron-doped diamond at around 2–2.5 V (vs Ag/AgCl).
#electrochemiluminescence, #Microscopy, Electrode, biosensors
Nano-immunoEra mid-term meeting in Rome
🌎Nano-immunoEra mid-term meeting in Rome: Happy to share some moments of the workshop organized by the UNITOV analytical group.
Nano-immunoEra workshop (14-17 April 2024 ) in Bologna
🌎Nano-immunoEra workshop (14-17 April 2024 ) at UNIBO: Happy to share some moments of the workshop organized by the UNIBO analytical group. The workshop “One health: new frontiers for bioanalytical chemistry” lasted two and a half days accompanied by a second event focused on “quality control workshop for the one health challenge: the quality of the data”. Particular attention was on the challenges of Data Management in One Health Model and the concept of FAIR data
Interview with Prof Alessandro Porchetta
New interview with Prof Alessandro Porchetta relating to the recent publication on Angew Chem.
https://onlinelibrary.wiley.com/doi/10.1002/anie.202319677
#Cas12, #CRISPR biosensors, #strand displacement reaction
New Paper in Angewandte Chemie International Edition
New paper in Angewandte Chemie International Edition
PAM-Engineered Toehold Switches as Input-Responsive Activators of CRISPR-Cas12a for Sensing Applications
Toehold Switch DNA presenting a locked anti-PAM in the loop are designed to control Cas12a activity in response to different molecular inputs. The hairpin to duplex reconfiguration triggered by co-localization-based displacement reactions provides a means to control the accessibility of the PAM and as a consequence Cas12a activity. We demonstrate the rapid, one-pot detection of antibodies and small molecules with high sensitivity and specificity.
#Cas12, #CRISPR biosensors, #strand displacement reaction