New Paper in Angewandte Chemie International Edition
New paper in Angewandte Chemie International Edition
PAM-Engineered Toehold Switches as Input-Responsive Activators of CRISPR-Cas12a for Sensing Applications
Toehold Switch DNA presenting a locked anti-PAM in the loop are designed to control Cas12a activity in response to different molecular inputs. The hairpin to duplex reconfiguration triggered by co-localization-based displacement reactions provides a means to control the accessibility of the PAM and as a consequence Cas12a activity. We demonstrate the rapid, one-pot detection of antibodies and small molecules with high sensitivity and specificity.
#Cas12, #CRISPR biosensors, #strand displacement reaction
Meet our secondees Elisa Paialunga
🌎Meet our secondees: Elisa Paialunga from 🇮🇹 (UNITOV) to 🇨🇭(CSEM). Elisa Paialunga will work on the design and fabrication of microfluidic systems through 3D printing.
🇮🇹 => 🇨🇭
Elisa Paialunga obtained a master’s degree in Chemistry from the University of Rome, Tor Vergata, under the supervision of Prof. Alessandro Porchetta. Currently, she is in the final year of her Ph.D. in Chemical Sciences & Technologies, under the supervision of Prof. Laura Micheli.
Her research focuses on the development of biosensors based on CRISPR-Cas for the detection of antibodies.
In Nano-ImmunoEra, she is actively involved in the design and fabrication of microfluidic systems through 3D printing, enabling the transposition of CRISPR-Cas assays into diagnostics in integrated devices.