New Paper in Angewandte Chemie International Edition

New paper in Angewandte Chemie International Edition

PAM-Engineered Toehold Switches as Input-Responsive Activators of CRISPR-Cas12a for Sensing Applications

Toehold Switch DNA presenting a locked anti-PAM in the loop are designed to control Cas12a activity in response to different molecular  inputs.  The hairpin  to  duplex  reconfiguration  triggered  by  co-localization-based  displacement  reactions  provides  a means to control the accessibility of the PAM and as a consequence Cas12a activity. We demonstrate the rapid, one-pot detection of antibodies and small molecules with high sensitivity and specificity.

#Cas12,  #CRISPR  biosensors, #strand displacement reaction

Paper: https://doi.org/10.1002/anie.202319677

Meet our secondees Elisa Paialunga

🌎Meet our secondees: Elisa Paialunga from 🇮🇹 (UNITOV) to 🇨🇭(CSEM). Elisa Paialunga will work on the design and fabrication of microfluidic systems through 3D printing.

🇮🇹 => 🇨🇭

Elisa Paialunga obtained a master’s degree in Chemistry from the University of Rome, Tor Vergata, under the supervision of Prof. Alessandro Porchetta. Currently, she is in the final year of her Ph.D. in Chemical Sciences & Technologies, under the supervision of Prof. Laura Micheli.

 

Her research focuses on the development of biosensors based on CRISPR-Cas for the detection of antibodies.

 

In Nano-ImmunoEra, she is actively involved in the design and fabrication of microfluidic systems through 3D printing, enabling the transposition of CRISPR-Cas assays into diagnostics in integrated devices.