PAM – Engineered Toehold Switches as Input-Responsive Activators of CRISPR-Cas12a for Sensing Application

Toehold Switch DNA presenting a locked anti-PAM in the loop are designed to control Cas12a activity in response to different molecular  inputs.  The hairpin  to  duplex  reconfiguration  triggered  by  co-localization-based  displacement  reactions  provides  a means to control the accessibility of the PAM and as a consequence Cas12a activity. We demonstrate the rapid, one-pot detection of antibodies and small molecules with high sensitivity and specificity.

#Cas12,  #CRISPR  biosensors, #strand displacement reaction

Electrogenerated chemiluminescence at boron-doped diamond electrodes

The paper presents Boron Doped Diamond as new electrode for Electrochemiluminescence:

Boron-Doped Diamond (BDD) as an electrode material for ECL which has complementary properties compared to the most common metals (e.g., Au or Pt) and carbon materials (e.g., glassy carbon, carbon nanotubes and graphene). Boron-doped diamond electrodes emerged as novel electrodes, gaining more and more interest from the electrochemical community for their peculiar characteristics such as a wide solvent window, low capacitance, resistance to fouling and mechanical robustness.

Nano-immunoEra workshop (14-17 April 2024 ) in Bologna

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Nano-ImmunoEra online Meeting 11/01/2024

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Nano-immunoEra Video is now online

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Nano-immunoEra workshop (13-15 December 2023 ) in Rome

🌎Nano-immunoEra workshop (13-15 December 2023 ) at UNITOV: Happy to share some moments of the workshop organized by the…

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Nano-ImmunoEra Kick-off Meeting

Feb 1st, 2023. kick-off meeting for the MSCA Staff Exchanges in Bologna: Nanotechnology enabled detection of clinically…

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